immunofluorescence icam 1 primary goat polyclonal r d system  (R&D Systems)

Journal: Scientific Reports

Article Title: Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation

doi: 10.1038/s41598-017-00418-y

Figure Lengend Snippet: Inflammatory cytokine expression is attenuated in AngII-Ald-infused PAI-1 −/− /uPA −/− mice. qRT-PCR ( a ) and Western blot ( b ) analysis of ICAM-1 expression in heart tissues at 4 wk of AngII-Ald infusion. In Western blot, densitometric analysis of relative ICAM-1 expression after normalisation with GAPDH is shown in the lower panel. Western blot images are cropped for concise presentation. All uncut western blot images are provided in Supplementary Figure . qRT-PCR ( c ) and ELISA assays ( d ) of KC expression in heart tissues at 4 wk of AngII-Ald infusion were performed as described.

Article Snippet: The membrane was immunoblotted with polyclonal goat-anti-mouse ICAM-1 (R&D Systems) overnight at 4 °C.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation

doi: 10.1038/s41598-017-00418-y

Figure Lengend Snippet: Loss of Pm activity results in reduced cardiac fibrosis in AngII-Ald infused mice. qRT-PCR ( a ) of transcripts of Col1a1, ICAM-1, TGF-β2, and MMP-2, Western blot analysis ( b ) of ICAM-1 expression, ELISA assays ( c ) of total and active TGF-β2 and MMP-2, and histological analyses ( d ) of Masson trichrome (collagen), hemosiderin deposition, and leukocyte infiltration (CD45 and Mac-3) at 4 wk of AngII-Ald infusion were performed as described (n = 3–4 mice per group). Western blot images are cropped for concise presentation. The uncut images are provided in Supplementary Figure .

Article Snippet: The membrane was immunoblotted with polyclonal goat-anti-mouse ICAM-1 (R&D Systems) overnight at 4 °C.

Techniques: Activity Assay, Quantitative RT-PCR, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay